Blasted Bioinformatics!?
Bioinformatics lessons learned the hard way, bugs, gripes, and maybe topical paper reviews too...
2025-05-20
What have you done to your keyboard?! Hands Down Promethium on a MacBook
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There were a few stickers on the lid, but why does my laptop look like this now? In short, I'm learning to touchtype a non-qwerty layou...
2024-06-07
FASTQ uploads to ENA FTP site with rclone
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I've recently been working what I considered to be a large scale FASTQ upload to the European Nucleotide Archive (ENA), from where it wi...
2024-02-02
BLAST max-target-seq meets metabarcoding
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This is my first blog post in years - primarily down to a second child who is now a toddler. And what better topic to return to than a mains...
2019-01-08
An overly aggressive optimization in BLASTN and MegaBLAST
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All my recent blog posts have been looking at issues raised by the recent letter Shah et al. (2018) "Misunderstood parameter of NCBI BL...
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2018-12-07
BLAST tie breaking by database order
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My November blog posts discussing the BLAST+ tools behaviour with an alignment limit setting (see What BLAST's max-target-sequences does...
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